---
title: "Impact of Diode Laser, Ozonated Water, and Chlorhexidine on Oral Microbiota: Insights from Proteomic Analysis"
description: Advanced proteomic study comparing diode lasers, ozonated water and 0.20% chlorhexidine to assess their effectiveness in selectively modulating the oral plaque microbiota.
image: https://blog.88dent.com/hubfs/Blue%20Modern%20Tropical%20Holiday%20Vacation%20Guide%20Blog%20Banner-Nov-13-2025-11-41-51-4068-AM.jpg
---
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# Impact of Diode Laser, Ozonated Water, and Chlorhexidine on Oral Microbiota: Insights from Proteomic Analysis
27 November, 2025
The human oral microbiota is a complex and dynamic ecosystem essential to maintaining oral and systemic health. Dysbiosis can contribute to a wide range of diseases.
Using a proteomic approach, this study investigates the effects of **diode laser**, **ozonated water**, and **0.20% chlorhexidine** on oral bacterial populations, highlighting their distinct influence on microbial balance and selectivity toward pathogenic species.
### Authors
**Ziba Najmi¹, Emanuele Ruga², Andrea Melle³, Rachele Pertusati³, Anna Maria Agnone⁴, Andrea Cochis¹, Alessandro Calogero Scalia¹\***
¹ *Department of Health Sciences, Center for Translational Research on Autoimmune and Allergic Diseases (CAAD), Università del Piemonte Orientale (UPO), Italia.*
² *DDS; MSc in Chirurgia Orale e Medicina Estetica; Specialista in Chirurgia Orale, SCDU Odontostomatologia, Osp. S. Andrea, Vercelli – ASLVC; Università del Piemonte Orientale (UPO), Italia.*
³ *RDH; MSc; SCDU Odontostomatologia, Osp. S. Andrea, Vercelli – ASLVC; Università del Piemonte Orientale (UPO), Italia.*
⁴ *DDS; Specialista in Chirurgia Orale; Professore a Contratto, Università del Piemonte Orientale; Divisione di Odontoiatria, Ospedale Sant’Andrea, Vercelli, Italia.*
\* *Corrispondenza: Alessandro Calogero Scalia, Department of Health Sciences, CAAD, Università del Piemonte Orientale (UPO), Italia.*
### Technological Note
The study was performed using the **PocketLaser (88dent, Italy)** — a 915 nm diode laser — to evaluate the antimicrobial effects on bovine dentin discs.
.
### The complete article describes in detail:
****
All treatments significantly reduce pathogens such as *Fusobacterium nucleatum* and *Prevotella oris*
**Diode laser** and **ozonated water** better preserve commensal species, supporting microbial balance (eubiosis).
Findings suggest the potential clinical use of laser and ozone as effective alternatives or adjuncts to chlorhexidine in oral disinfection and periodontal therapy.
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